Much work remains to be achieved before sulforaphane can be tested clinically with EBS individuals, but Coulombe notes that extracts from broccoli sprouts rich in sulforaphane have already been shown to be safe for use in human skin. In EBS patients, underneath layer of the epidermis, which is made from cells called keratinocytes, is normally fragile and ruptures readily unusually. Molecularly, most instances of EBS result from mutations in genes that make the proteins keratin 5 and keratin 14 . These proteins co-polymerize to create the intermediate filament cytoskeleton in basal keratinocytes. Because the discovery in 1991 that EBS is a keratin-based disease, more than 40 additional disorders affecting a broad range of tissues have already been traced to defects in genes that encode intermediate filament proteins.Oakland officials are seeing it differently, however. CBS SAN FRANCISCO BAY AREA states: Pleasant Grove Baptist Church in West Oakland lately received a letter from the city threatening a $3,500 nuisance fee and a $500 a day fine unless it toned down its choir practice. The letter states in part, ‘. This activity may constitute a public nuisance due to its impact to the use and quiet enjoyment of the encompassing community’s property.’ Put simply, the First Amendment rights of Pleasant Grove should be trampled on to be able to ensure that other people who live nearby are guaranteed a right that’s not detailed in the Constitution: a right to ‘quiet.’ ‘Kind of hard to trust because we have been here about 65 years in the community and suddenly we get some concerns about the sound,’ Thomas A Harris III, the pastor at Pleasant Grove, told the neighborhood affiliate marketer.